Abstract 5914: E-cadherin and beta-catenin in oral dysplasia progression
Notice bibliographique
Résumé
Abstract Objectives: Epithelial-mesenchymal transition (EMT), a biological process characterized by a progressive decrease in epithelial features and increase in mesenchymal traits, has been suggested as a critical mechanism in cancer development. A defining feature of EMT is the loss of E-cadherin. Together with beta-catenin, alpha-catenin, and p120-catenin, E-cadherin forms the adherens junctions, which are involved in cell-cell adhesion of epithelial cells. As beta-catenin has both a structural and signaling role, E-cadherin potentially contributes to malignant progression via beta-catenin signaling through the Wnt pathway. E-cadherin and beta-catenin expression is altered from normal oral tissue, oral epithelial dysplasia (OED), to oral squamous cell carcinoma (OSCC), but there is no longitudinal research on the role of these biomarkers in malignant progression. The objective of this study is to explore the expression of E-cadherin and beta-catenin in OED and to determine if these expression patterns predict malignant progression. Methods: 28 progressors (PR) and 56 non-progressors (NPR) were included in this case-control study. Samples that progressed to severe OED, carcinoma in situ, or OSCC were categorized as PRs, and samples that did not progress were NPRs. Patient samples with a baseline biopsy of low-grade (mild or moderate) OED and at least 5 years of follow-up for NPRs were obtained from the Oral Cancer Prediction Longitudinal study, which has been prospectively following patients with OED for over 20 years. Immunohistochemistry (IHC) is performed on formalin-fixed paraffin-embedded tissue samples to assess for reduced membranous E-cadherin, and reduced membranous and increased cytoplasmic and/or nuclear beta-catenin expression in PRs compared to NPRs. We hypothesized that OED with this expression pattern will show greater likelihood of progression. Fisher’s exact test was used. Logistic regression will be performed to predict progression. Results: There were no significant differences in age, sex, risk of lesion site, and grade of dysplasia between PRs and NPRs. There were significant differences in length of follow-up time and smoking history. IHC has been completed on 27 samples to date, which show decreased membranous beta-catenin expression in PRs compared to NPRs (p=0.042). IHC for additional samples will be performed to assess for cytoplasmic and nuclear beta-catenin expression, and to compare expression patterns between PRs and NPRs to determine prediction of malignant progression. Conclusion: Early results suggest that membranous beta-catenin expression may be decreased in OED that progressed compared to OED that did not. This research enhances the understanding of EMT’s role in malignant progression and hopes to potentially aid in the intervention of oral lesions at risk of cancer. Citation Format: Ilena Yim, Iris Lin, Leigha Rock, Lewei Zhang, Miriam Rosin, Denise Laronde. E-cadherin and beta-catenin in oral dysplasia progression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5914.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,004 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».