Accelerating biophysical studies and applications by label-free nanopore sensing
Pourquoi ce travail est dans la base
Une base qui oublie comment elle a trouvé un travail ne peut pas être vérifiée. Voici les voies qui ont admis celui-ci.
Notice bibliographique
Résumé
Label-free single-molecule sensing technologies are attractive tools for investigating the properties of biological molecules via the understanding of molecular functionality.Among these technologies, nanopore sensing has become one of the growing technologies [1,2].Nanopore sensing operates in the principle of resistive pulse sensing, where sensing molecules, such as DNA, RNA, and protein, pass through a pore under the electrical field, resulting in a blockade current due to the molecular occupation in a pore.The physical properties of sensing entities were obtained by analyzing blockade current, which can provide a fingerprint of sensing molecules (Figure 1) [3].In this commentary article, we review the eight presentations at the symposium "Innovative label-free nanopore sensing toward biophysical studies and applications" of the 60th Annual Meeting of the Biophysical Society of Japan held in September 2022 and introduce how this sensing technology can be used as a tool to open new biophysical science or applications other than DNA sequencing.Kyle Briggs at Ottawa University/ Northern Nanopore Instruments talked about an automated method of electricalbased nanopore fabrication, which is one of the gold standard fabrication methods in the lab, and introduced how to accelerate solid-state nanopore research using this method [4,5].He also presented the automated muti-pore fabrication tools having multi-channels fluidic flow cells with multi-membrane chips.Finally, he showed the nanopore trace analysis software, Nanolyzer, which has multiple functions such as multi-level blockade current fitting, overlay translocation events, kernel density estimation, etc.Kan Shoji at Nagaoka University of Technology presented a probe-type planer bilayer lipid membrane (pBLM) system [6,7] and its application for scanning ion conductance microscopy (SICM) [8].In this system, pBLMs can be repeatedly formed at the tip of probes by inserting probes into a layered bath solution of an oil/lipid mixture and electrolyte.He mounted the probe into a SICM setup and demonstrated spatially-resolved chemical sensing by manipulating the probe.Additionally, he introduced an efficient current measurement system for synthetic DNA nanopores.Although DNA nanopore structures are expected to be applied for nanopore sensing, it is challenging to efficiently insert DNA nanopores into pBLMs.He prepared DNA nanopore-tethered gold electrodes and formed pBLMs on the surface of electrodes by inserting electrodes into the bath solution.Resultantly, efficient insertions of DNA nanopores were observed, and this method potentially accelerates applications of DNA nanopores for nanopore sensing.Figure 1 The fundamental working principle of nanopore sensing
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découle