A231 THE FLAVANOID CYANIDIN REDUCES INTESTINAL INFLAMMATORY DAMAGE BY MAINTAINING INTESTINAL PERMEABILITY
Notice bibliographique
Résumé
Abstract Background Since the discovery and the use of proton pump inhibitors (PPI), the incidence of gastric ulcers has reduced, however, there has been an increase in intestinal lesions since PPIs do not guarantee protection to the intestine. To protect the deeper layers of the intestinal wall, the intestinal barrier tightly regulates the passage of pro-inflammatory molecules, microorganisms, toxins, and antigens. The epithelium is damaged during intestinal inflammation due to the action of inflammatory cytokines such as TNF resulting in ulceration. Cyanidin is a flavonoid of the anthocyanin class, found in several red fruits, with anti-inflammatory and antioxidant activity. Purpose We hypothesized that the administration of cyanidin can decrease the deleterious effects caused by polypharmacy (PPI + NSAID) in the small intestine by reducing intestinal permeability. Method In vivo: Male mice (8 weeks old) were treated to induce intestinal ulcers caused by polypharmacy (combination of PPI - lansoprazole 20 mg/kg daily, non-steroidal anti-inflammatory drug (NSAID) - acetylsalicylic acid 10 mg/kg daily and COX-2 selective NSAID, celecoxib 20 mg/kg daily). On the ninth day, oral treatment with cyanidin (5 mg/kg) or vehicle was started until the fourteenth day, then the animals were killed for parameter analysis lesion. ELISA was performed to quantify interleukins (IL-10, IL-6, IL-1β) and cytokine (TNF), and we assessed the antioxidant profile (SOD, CAT, and GSH) and measured gene expression of TNF, IL-10, IL-6 TRL-4, HMOX-1, MMP 2 and 9, COX-1, MUC-3, ZO-1, CL-1. In vitro: Monolayers of colonic epithelial cell lines (Caco-2 at 21 days of confluence) were mounted in Ussing chambers to assess barrier function and to determine transepithelial resistance (TER). To analyze the permeability response to injury, we utilized TNF and IFN (25 ng/mL) with cyanidin (10 or 100 uM) for 48 h in transwell plates, with measurement of total intestinal permeability using 4 kD FITC-dextran. Result(s) Analysis of mouse intestine indicated that cyanidin (5 mg/kg) significantly reduced expression of IL-6 and TNF, TLR4, and HMOX-1 (p<0.05), and increased gene expression of MUC-3, CL-1, occludin, COX-1, and IL-10 (p<0.05) without altering antioxidant parameters. Cyanidin (100 mM) maintained barrier function as shown by transepithelial electrical resistance (TER), and also significantly reversed the detrimental effects of the inflammatory cytokine on FITC-dextran flux in Caco-2 cells (p<0.05). This may be related to the increased expression of occludin and ZO-1 in the intestinal epithelium of mice. Conclusion(s) These in vivo and in vitro results suggest that cyanidin decreases the polypharmacy-induced intestinal inflammatory response while maintaining the integrity of the intestinal epithelium. Other trials are underway to elucidate these mechanisms. Please acknowledge all funding agencies by checking the applicable boxes below Other Please indicate your source of funding; NSERC; FAPESP Disclosure of Interest None Declared
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,007 | 0,004 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,002 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».