P0018 PP AMELIORATING SMALL BOWEL INJURY USING A CAVITARY TWO‐LAYER PRESERVATION METHOD WITH PERFLUOROCARBON AND A NUTRIENT‐RICH SOLUTION
Notice bibliographique
Résumé
Introduction: Small bowel transplantation is currently used as a therapeutic option in selected cases of permanent pediatric intestinal failure who experience life-threatening complications from parenteral nutrition such as end stage liver disease, lack of venous access and IV line infection. On a worldwide basis two out of every three SBT have been performed in children under 18 years of age. The success rate of SBT is quite low (<40% 5-year survival). A major obstacle to successful small bowel transplantation is that of bacterial infection as a direct result of bacterial translocation across an injured bowel graft related to preservation. The aim of this study was to improve graft quality during cold storage by combining two proven preservation strategies involving perfluorocarbon and a novel luminal preservation solution. Methods: Small bowel from Sprague-Dawley rats (n=30) was flushed vascularly with modified UW solution and flushed luminally as below: Group 1- none (control); Groups 2 and 3 – UW solution; Groups 4 and 5 – an amino-acid rich solution (a mixture of 19 amino acids based on physiological requirements). Groups 2–5 were then submersed in perfluorodecalin (pre-saturated with oxygen). In Groups 3 and 5, perfluorocarbon solution was bubbled continuously with 100% oxygen at a rate of 50 to 100 mL/min. Energetics, oxidative stress and histology were assessed over 24 h at 4oC. Results: ATP levels were consistently greater than control throughout 24 h in Groups 2 to 5. Of particular clinical relevance, Group 4 exhibited significantly greater ATP and ATP/ADP ratios and energy charge level after 12 h storage; values were almost 2 fold greater than values in other Groups. Even after 24 h Group 4 energetic values was significantly higher than those of other groups indicating a greater reserve of high-energy molecules and, thus, better preserved tissue. In terms of lactate accumulation, levels of this end product were elevated in Groups 3 and 5 compared with other groups during the entire 24 h. Overall, histologic injury was lower in amino acid treated tissues than others; after 12 h storage median injury in Groups 4 and 5 indicated villus denudation with only 1/6 specimens exhibiting evidence of crypt infarction. Histologic injury was more prevalent in Groups 1, 2 and 3 with a greater incidence of crypt and transmucosal infarction. Even after 24 h, Groups 4 and 5 showed superior mucosal integrity. Conclusion: Our data indicate that combined treatment with perfluorocarbon and luminal amino acid–rich preservation solution significantly improves energetics and histologic integrity. This combined strategy may have implications for the successful preservation and transplantation of SB in the clinic.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».