262 Modulation of vitamin D, calcium and phosphorous metabolism and immune response in pre- and post-weaning piglets under different vitamin D supplementation strategies
Notice bibliographique
Résumé
Abstract This study evaluated the impact of different vitamin D supplementation strategies to pre- and post-weaning piglets on vitamin D, calcium and phosphorous metabolism and immune status. Sixty piglets were selected at d 2 of age [body weight (BW) = 1.71 ± 0.03 kg] and orally supplemented with saline (CTR) or a solution of 25(OH)D3 at d 2, d 8, and d 21 (0.1, 0.2 and 0.3 mg, respectively) along with UVB exposure every second day from d1 4 up to d 21 (HD). From weaning (d 21) until d 42, CTR animals were supplemented with 2,000 UI of vitamin D3/kg of feed and HD animals were supplemented with 2,000 UI 25(OH)D3/kg. Piglets were sacrificed at d 21, d 28, and d 35 (n = 10/treatment/d) for the collection of blood, jejunum, liver, and kidney samples. Serum 25(OH)D3 concentrations were determined by HPLC and the mRNA expression of genes implicated in vitamin D, calcium and phosphorous metabolism and immune status were assessed by quantitative real-time PCR. Data were analyzed using a 2x3 factorial design. Serum 25(OH)D3 concentrations were 2.8, 3.6, and 2.2-fold greater in HD piglets at d 21, 28, and 35 (P ≤ 0.01), respectively (Table 1). No treatment or treatment x time interaction was detected on the expression of genes related to vitamin D metabolism in the jejunum mucosa and liver (P ≥ 0.19). In kidney, HD piglets had greater expression of CYP27B1 (P = 0.09) and decreased expression of CYP24A1 (P = 0.06). Renal EGR1 expression was greater in HD piglets (P = 0.01). No treatment effect was detected on the expression of genes directly implicated in phosphate or calcium reabsorption (P ≥ 0.16). No treatment or treatment x time interaction was detected on the mRNA expression of selected genes related to immune response in the jejunum mucosa, liver, and kidney (P ≥ 0.14). Independently of vitamin D treatments, time effects were detected for gene expression. The mRNA expression of CYP27B1 increased and that of CYP24A1 decreased from d 28 to 35 (P ≤ 0.04) in kidney. Genes related to calcium (TRPV5) and phosphate (NPT2c) reabsorption in kidney had reduced mRNA expression on d 35 compared with d 21 (P ≤ 0.01). Overall, the mRNA expression of genes related to immune response in all studied tissues was lower at d 35 compared with d 21 (P ≤ 0.08). Bodyweight at slaughter was not affected by treatments at d 21, 28, and 35 (P ≥ 0.29). In conclusion, pre-weaning vitamin D supplementation enhanced vitamin D status at weaning and the use of dietary 25(OH)D3 supplementation was more effective in increasing the vitamin D status post-weaning. The mRNA expression results suggest that greater vitamin D status at weaning and during the first 2 wk post-weaning do not affect calcium and phosphate reabsorption in kidney and the immune defense of pre- and post-weaning piglets.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».