Unique findings of <i>Phoma</i>-like fungi associated with soybean
Notice bibliographique
Résumé
Ascochyta leaf blight of soybean is a widespread disease caused by several closely related Phoma-like species, this disease often leads to significant crop losses. Among Phoma-like species from Didymellaceae family, the most frequently associated with symptomatic soybean tissues are species of the genera Boeremia and Didymella. Currently reliable species identification in Didymellaceae relies on polyphasic approach based on consolidated species concept and combined molecular phylogenetic, micromorphological and cultural features. At least three loci are commonly used for reconstruction of the molecular phylogeny of Didymellaceae: internal transcribed spacer (ITS) of the ribosomal DNA, partial RNA-polymerase II gene (rpb2), and β-tubulin (tub2). As a result of long-term phytosanitary monitoring of soybean crops, soybean leaves with symptoms of Ascochyta blight were collected from major soybean producing areas of Russia. From surface sterilized plant tissues more than 100 isolates of Phoma-like fungi were obtained and stored in the collection of pure cultures of the Laboratory of Mycology and Phytopathology (MF, All-Russian Institute of Plant Protection). Most of them, as a result of multilocus phylogenetic analysis, were identified as Boeremia and Didymella species. Eight isolates were identified as species of other genera, suspected to be rare findings. The aim of this study was to identify these eight isolates based on multilocus phylogenetic analysis, as well micromorphological, cultural, and pathogenicity data. Multilocus phylogenetic analysis has resulted in identification of all eight isolates to species level. Single isolate from the Ryazan region was Neoascochyta graminicola. Three other from three different districts of the Amur region were Remotididymella capsici. Two isolates from the Primorskiy territory and Amur region were Stagonosporopsis heliopsidis. Another two from two districts of the Amur region were S. stuijvenbergii. Pathogenicity tests have resulted in conclusion, that all studied isolates were not pathogenic for soybean leaves. Probably, these Phoma-like species are associate with soybean as saprophytes or endophytes. For all these Phoma-like species Glycine max was detected as substrate for the first time. Neoascochyta graminicola is widespread in Europe in association with Poaceae plants. There are only two findings of Remotididymella capsici in the world, both from leaves of Capsicum annuum. First finding was made in the former USSR in 1977 and was identified based on only morphological features. Second findings was collected in the Fiji and verified with multilocus phylogenetic analysis. Stagonosporopsis heliopsidis isolates were revealed in the USA, Canada, Netherlands and Russia and this fungus was believed to be specific for Asteraceae plants. Isolates of Stagonosporopsis stuijvenbergii are known only from soil in the Netherlands. Thus, such species as Neoascochyta graminicola and Stagonosporopsis stuijvenbergii were revealed in the Russia for the first time. Studied Remotididymella capsici isolates were first confirmed findings of this fungus in Russia. Additionally to detailed phylogenetic data, the manuscript is supplement with a detailed description of the cultural and micromorphological features of all species.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».