MALDI-TOF lipidomics rapidly detects modification of 2-hydroxymyristate lipid A, a potential virulence trait in <i>Enterobacter bugandensis</i>
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Résumé
ABSTRACT Enterobacter cloacae complex (ECC) comprises numerous species that can be difficult to identify. Among these species, Enterobacter bugandensis is recognized as a threat in neonatal intensive care units due to its involvement in septic shock. A potential virulence factor responsible for its virulence is the modification of the lipid A of the lipopolysaccharide (LPS). This modification corresponds to a hydroxylation of the myristate component of lipid A. A collection of 168 ECC, including 10 E. bugandensis and one isolate involved in fatal septic shock in the neonatal intensive care unit, were tested for the presence of 2-hydroxymyristate. All isolates were sequenced to determine the genetic diversity of this collection and detect the presence of the lpxO gene responsible for this hydroxylation. matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) analysis revealed that only a fraction (4/11) of E. bugandensis were able to modify their lipid A, which correlates with the presence of the lpxO gene. Genomic analysis of E. bugandensis revealed that only half of the genomes carried the lpxO gene. Lpxo-negative isolates exhibited a deletion of 1,094 bp encompassing the lpxO gene. Lipid A analysis also revealed that hydroxylation of myristate was not specific to E. bugandensis since this modification was systematically found in several species including Enterobacter chengduensis , Enterobacter chuandaensis, or Enterobacter cloacae subsp cloacae. Here, we provide a simple detection test allowing the rapid identification of hydroxymyristate of lipid A involved in the virulence of E. bugandensis . Rapid identification of lipid A modification might help clinicians to adapt procedures when an Enterobacter spp. is identified in newborns. IMPORTANCE Enterobacter bugandensis is now recognized as a potential threat in neonatal wards due to its ability to survive in incubators and its virulence properties. Its increased virulence is linked to the addition of 2-hydroxymyristate on lipid A, resulting from the action of the LpxO enzyme. Here, we proposed a rapid and simple assay to decipher the presence of 2-hydroxymyristate on lipid A directly from the bacterial colony. This assay can be performed easily on MALDI-TOF routine machine using a commercial kit (MBT Lipid Xtract Kit, Bruker Daltonic). This test can accurately detect the 2-hydroxymyristate on lipid A of lpxO-positive ECC isolates, including E. bugandensis . In neonate wards, newborns are now screened for ECC carriage. Accordingly, the MALDIxin test might help clinicians detect the presence of lpxO-positive E. bugandensis, which can be considered a risk factor for the newborn, and implement measures to avoid potentially fatal septicemia.
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Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découle