Establishing microbial communities to promote the growth of <i>Pleurotus ostreatus</i> through a top-down approach is hindered by the dominance of antagonistic interactions
Notice bibliographique
Résumé
ABSTRACT Few attempts have been made to examine the diversity and functions that characterize microbial communities compatible with and beneficial to the biotechnologically relevant mushroom Pleurotus ostreatus . The quest for complementarity is complicated by the variable nature of beneficial traits, impairing the rational assembly of synthetic communities to improve bioprocesses. This study investigated whether the compatibility between P. ostreatus and microbial enrichments is lessened in conditions favoring saprophyte metabolism and is enhanced when a combination of recalcitrant and labile carbon is integrated in the enrichment. The microbial diversity of enrichment cultures and substrates colonized by P. ostreatus was analyzed through PCR amplicon sequencing, and the proliferation of P. ostreatus was assessed by quantitative image analysis. In general, co-inoculation of lignocellulosic substrate with microbial enrichment reduced the growth of P. ostreatus . The saprophytic enrichment conditions were more conducive to the development of antagonistic communities inhibiting P. ostreatus growth than enrichment cultures integrating labile carbon substrates. Both microbial community analyses and in vitro assays led to the identification of a single phylotype affiliated with Brevundimonas spp., which displayed neutral interaction with P. ostreatus . Recalcitrant and labile carbon degradation functions were not primary factors driving beneficial microbial communities for P. ostreatus . Additional functions beyond carbon metabolism are likely to promote beneficial interactions. Directed enrichment cultures, integrating the mushroom in the earliest stage of the procedure, are expected to promote more beneficial interactions than top-down approaches. IMPORTANCE Lignocellulosic biomass upcycling biotechnologies integrating solid-state fermentation by fungi are aligned with sustainable development perspectives. While the recalcitrance of this biomass imposes a challenge for the implementation of these bioprocesses converting the lignocellulosic feedstock into bioenergy and bioproducts, pretreatment of lignocellulose biomass with fungi is efficient and generates fewer by-products than chemical approaches. Optimization and stabilization of this bioprocess by integrating microbial consortia has received little attention. The significance of our research is to bridge that knowledge gap by examining how interactions between the biotechnologically relevant basidiomycete Pleurotus ostreatus and microbial communities influence fungal growth in lignocellulosic substrate. Directed enrichment cultures integrating Pleurotus ostreatus as a selective agent are expected to trigger more beneficial interactions promoting mushroom growth than our top-down approaches, due to a dominance of antagonistic mushroom-bacteria interactions.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».