Pathways of neutrophil enzymatic degradation of resin-based composites and adhesives
Notice bibliographique
Résumé
Human Neutrophils (HN), white blood cells of the innate immune system, are abundant inhabitants of the oral cavity. It was previously found that these leukocytes can degrade methacrylate resin-based composites (RC), adhesives, monomers, and demineralized dentin. This activity can compromise the tooth-restoration interface, potentially resulting in restoration failure and debonding. Nevertheless, the exact mechanism of this activity is yet to be fully determined. The objectives of this study were to measure cluster of differentiation (CD) marker expression and cell viability of HN when exposed to methacrylate resin-based monomers, degradative byproducts, RC, and adhesives, then to measure and analyze neutrophil-derived enzymes’ degradative activity towards methacrylate resin-based materials. HN’s CD marker expression in the presence of methacrylate resin-based monomers, degradative byproducts, composites, and adhesives was quantified via flow cytometry analysis using a panel of 7 CD markers. HN and neutrophil-derived enzyme degradation of methacrylate resin-based materials was analyzed using ultra performance liquid chromatography and mass spectrometry. Lastly, in silico 3D characterization of the neutrophil-derived enzymes catalytic site was conducted. Results showed increased CD66a among HN exposed to RC and universal-etch adhesive (UE), and upregulated CD11b and CD18 following exposure to UE. Whole HN and neutrophil elastase (NE) degraded 2,2-Bis [4-(2-hydroxy-3-methacryloxypropoxy) phenyl] propane (bisGMA) and urethane dimethacrylate (UDMA) monomers, and photocured RC and UE dental materials(p>0.05). In silico 3D characterization of NE’s active site revealed selective binding configurations to bisGMA and UDMA monomers. These findings suggest that neutrophil-mediated enzymatic degradation of methacrylate resin-based materials may be an important contributing factor to restoration failure and recurrent caries. Neutrophils, innate immune system cells, are found in the gingival sulcus, adjacent to the margins of methacrylate resin-based composites and adhesives. There, neutrophils can combat pathogenic oral bacteria as the body’s first line of defense. This study reveals an in-depth mechanism of the neutrophil-derived enzymatic degradation of methacrylate dental materials, with neutrophil elastase being a key contributor to this potentially harmful activity. This activity, that can occur at the tooth-restoration interface found in the margins of the restorations, can contribute to premature failure of dental restorations, a multi $B health concern. The new culprits unveiled in this study can be targeted in preventive dental treatment, as well as when developing new dental materials with improved longevity and durability. Neutrophils, innate immune system cells, are found in the gingival sulcus, adjacent to the margins of methacrylate resin-based composites and adhesives. There, neutrophils can combat pathogenic oral bacteria as the body’s first line of defense. This study reveals an in-depth mechanism of the neutrophil-derived enzymatic degradation of methacrylate dental materials, with neutrophil elastase being a key contributor to this potentially harmful activity. This activity, that can occur at the tooth-restoration interface found in the margins of the restorations, can contribute to premature failure of dental restorations, a multi $B health concern. The new culprits unveiled in this study can be targeted in preventive dental treatment, as well as when developing new dental materials with improved longevity and durability.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».