Treating a C5 contusion injury in female rats with sigmoid protein and 4-methylumbelliferone in combination with single pellet grasping training
Notice bibliographique
Résumé
STUDY PURPOSE: We tested the effect of 4-methylumbelliferone (4-MU), an inhibitor for hyaluronan (HA) synthesis and Intracellular Sigmoid Protein, a PTP sigma antagonist, in combination with single pellet grasping (SPG) training on neuroplasticity and functional recovery following cervical spinal cord injury in rats. DATA COLLECTED: All rats (n=30) were trained on single pellet grasping (SPG) using semi-automated dispensers. Paw preference was determined within the first week of training. After a plateau was reached in both single pellet reaching attempts and success rate, all rats received unilateral cervical spinal contusions (based on paw preference) at C5 using the Infinite Horizon impactor. Rats were assigned to 2 groups based on the severity of the lesion, as determined by the cylinder test on day 3 post SCI. Four rats were excluded (n=2 failed to learn SPG, n=1 no discernible deficit, n=1 euthanised due to health issues). The Control group (n=13) consisted of animals who received 1 ml saline (s.c.) daily and nutella twice/day for 5 days/week for a total of 8 weeks. The 4MU+ISP group (n=13) received 500µg intracellular sigmoid protein daily (s.c.) and a total of 0.5g/Kg 4 methylumbelliferone (MU), 5 days a week in nutella. Treatments started at week 18 post SCI and continued through week 25 post SCI, for 8 weeks in total. SPG training started 18 weeks post injury (5x/week for 10 min each) for twelve weeks. Every 2 weeks during the training/treatment the rats were tested on the horizontal ladder. The treatments were stopped after 8 weeks and the rats were tested with cylinder, elevated plus maze, Irvine, Beatties and Bresnahan (IBB) Forelimb Scale, Forelimb Locomotor Score (FLS) and horizontal ladder tests. AAV9 tracering was administered bilaterally into the forelimb motor cortex to trace lesioned and unlesioned corticospinal tract (CST) tracts. The rats were euthanized 3 weeks after tracer injection. Livers were dissected and weighed, the brain and spinal cord cryoprotected for histologic examination. The spinal cord tissue damage was determined from GFAP immunostained serial horizontal section images by measuring the total area of damage. Analysis of collateral sprouting for both lesioned and unlesioned CST tracts, including the number of CST collaterals and the distance of collateral sprouting both rostral and caudal to the lesion from transverse sections. DATA USAGE NOTES:
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,002 | 0,002 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,002 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».