Molecular Cloning and Expression Characteristics of otx2 and eya1 During the Early Development of Seriola aureovittata
Notice bibliographique
Résumé
Yellowtail kingfish (Seriola aureovittata) is a pelagic migratory fish species with a global distribution. In recent years, interest in the aquaculture of this species has increased worldwide because of its high flesh quality and fast growth. As a large and fast-swimming pelagic fish, yellowtail kingfish has good adaptability to land-based industrial recirculating and offshore net cage modes. With the development of aquaculture technology, more attention has been paid to animal behavior research, which will promote the welfare level in aquaculture. Light, sound, temperature, density, and flow velocity are important factors affecting the welfare of farmed fish. The development, morphological structure, and regulatory mechanism of sensory organs, including visual, gustatory, olfactory, and auditory organs, are becoming increasingly important in the study of animal behavior.Orthodenticle homeobox 2 (OTX2) and eye absent 1 (EYA1) play important roles in regulating the ontogeny, differentiation, and development of visual, gustatory, olfactory, and auditory organs. In order to study the expression characteristics of otx2 and eya1 in yellowtail kingfish during early development, otx2 and eya1 were identified from brain tissue with specific kits. The lengths of otx2 and eya1 open reading frame domain were 876 and 1, 962 bp encoding 291 and 653 amino acids, respectively. Among these, OTX2 consists of a homologous domain from the 42nd to 93rd amino acids and a TF-Otx domain containing 82 amino acids; the C-terminal of EYA1 is an EYA domain encoding 246 amino acids. These two genes had a wide range of tissue expression characteristics, including in eye, brain, pituitary, head kidney, heart, liver, spleen, kidney, mid-gut, and ovary tissues. The highest expression level of otx2 was obtained in eye tissues followed by brain tissues, and the values in these two tissues were significantly higher than those in other tissues (P < 0.05). The highest expression level of eya1 was obtained in pituitary tissues followed by ovary tissues, and the values in these two tissues were significantly higher than those in other tissues (P < 0.05). During embryonic development, expression of otx2 and eya1 could be detected at each stage, and levels were upregulated at a late stage, during which otx2 and eya1 reached peak values at hatching stage and when the embryo encircled 4/5 of the yolk sac, respectively. At the larval and juvenile stages, the expression of otx2 and eya1 could be detected, with high expression levels at the early stage, during which the expression level of otx2 was first upregulated and then downregulated; the expression level at 3–25 dph (days post-hatching) was significantly higher than those at 1 dph and 30–60 dph, with the highest level reached at 20 dph (P < 0.05). The expression of eya1 showed a downward trend, with the highest level reached at 1 dph (P < 0.05); the expression levels at 30–60 dph were significantly lower than those at 7–25 dph (P < 0.05). This study provides a molecular basis for understanding the physiological functions of otx2 and eya1 during the development of sensory organs and the regulatory mechanisms in yellowtail kingfish.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».