Microplastics in American lobster (Homarus americanus) and Mussel Species in Coastal Southwestern New Brunswick, Canada
Notice bibliographique
Résumé
This dataset consists of data collected by the Passamaquoddy Recognition Group Inc. (PRGI), which is an Indigenous not-for-profit organization led by the Peskotomuhkati Nation at Skutik. This project is funded by the Department of Fisheries and Oceans (DFO), Coastal Environmental Baseline Program (CEBP). Data will include samples collected from 2020-2027 and represents field sampling data as well as laboratory and Fourier Transform Infrared Spectroscopy (FTIR) data associated with the sampling of American lobster (Homarus americanus) and Northern Horse mussel (Modiolus modiolus) for the purpose of studying the presence and abundance of microplastics and micro-debris in this species. Sampling: Samples were collected from the Port of Saint John region, Passamaquoddy Bay and around Grand Manan Island in New Brunswick. Samples were collected using standard lobster traps with PRGI technical staff present for all sampling activity. Retained lobsters were transported back to laboratory spaces in coolers and immediately euthanized via freezing. Laboratory Analysis: Once lobsters are euthanized and dissected, digestion of organic tissue, and filtration begin. Digestive tracts and muscle tissues are dissected from the lobster, and whole mussels are placed in a 10% KOH solution. Samples are then transferred into a warming oven at 50 degrees Celsius for at least 24 hours. This process allows for the digestion of most organic material. Large undigested remains are passed through a sieve and analyzed visually by technicians under a dissection microscope. Any potential microplastics identified at this stage are isolated on a filter paper and stored on a petri slide. The remaining tissue is filtered using a 0.8 µm pore size filter and a vacuum pump. As with larger debris, these filters are also analyzed visually under a dissection microscope. Any potential microplastics are marked and numbered for FTIR identification. Dissection, digestion, and visual analysis are conducted in a lab space, making use of biosafety cabinets, and fume hoods to aid in the reduction of microplastic contamination of samples, whenever possible. All implements used are triple rinsed with filtered (0.8 µm or Milli-Q) water, and samples are covered when not being handled to reduce potential contamination. Environmental blanks, H20 blanks, and KOH blanks are taken throughout the process for quality control. As of 2022, PRGI conducts laboratory analysis in a dedicated in-house clean lab, minimizing exposure to contaminants by following cleaning procedures and low plastics laboratory dress code. Once visual analysis is completed, petri slides with marked and numbered potential plastics are sent to Western Surface Science laboratory at the University of Western Ontario for FTIR analysis. This analysis indicates the chemical composition of identified particles and indicates if such particles are made of plastic or of other materials.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».