Investigating the lipidome of the lateral ventricle choroid plexus during ageing and across sex
Notice bibliographique
Résumé
During ageing, the brain's lipid composition and the cerebrospinal fluid (CSF) secreted by the choroid plexus (ChP) undergo significant modifications. The ChP, an epithelial tissue in each brain ventricle, experiences a decline in its functions, including protein secretion and CSF production. A crucial gap in understanding the ChP lies in its lipid composition and the potential repercussions of these changes on ChP physiology during ageing. This study hypothesised that the lipidome of the lateral ventricle choroid plexus (LVCP) is modulated during ageing, potentially generating pro-inflammatory mediators. The objective was to perform quantitative lipidomics on mouse LVCP during ageing and investigate whether pro-inflammatory lipid mediators increase as the mice age. LVCP and cortex tissues from mice aged 6, 12, 18, and 24 months (n = 5/age/sex) were collected from C57BL/6 mice of the Quebec Network for Research on Ageing. These tissues were analysed using liquid chromatography coupled with tandem mass spectrometry, and statistical analyses were conducted using the Lipidr software package and univariate statistical methods. During ageing, fifteen lipids in the LVCP showed significant changes: eight on a phosphatidylethanolamine (PE) backbone and six on a phosphatidylcholine (PC) backbone. Four lipids containing eicosapentaenoic acid were more abundant in 6-month-old mice than in 18-month-old mice, indicating a decrease in precursors of anti-inflammatory lipid mediators during ageing. No significant changes were observed in the lipids of 12-month-old mice compared to other ages. Phospholipids containing eicosapentaenoic acid were the most modified during ageing, with all significantly changed phospholipid species having lower concentrations in 18-month-old mice. The LVCP of males and females exhibited thirty-three lipids with distinctive profiles between sexes, notably in sphingomyelin, which were less concentrated in males. Additionally, there was a sex-by-age interaction for seven lipid mediators, six derived from linoleic acid. Contrary to the hypothesis, the lipidome of the LVCP did not favour a pro-inflammatory lipid profile or mediator profile during ageing, suggesting that the LVCP does not contribute to the synthesis of pro-inflammatory lipid mediators released in the brain during ageing.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».