Visceral distribution of the type II sodium‐dependent phosphate cotransporter (NaPi‐II) isomer mRNA and the expression of NaPi‐IIc mRNA along the intestinal longitudinal axis in the post‐weaned pig
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Molecular physiology of phosphate cotransporter expression in pigs.
It measures phosphate transporter expression in pigs, not research practice.
Biomedical localization of phosphate cotransporters; physiology, not metaresearch.
Résumé
The solute carrier family SLC34 includes the sodium‐phosphate cotransporter (NaPi) type IIa, b, c isomers in rodents and humans. The objective of this study was to identify the NaPi‐II isomers in porcine viscera and to quantify the NaPi‐II isomer mRNA in the gut of the weaned Yorkshire pig. We determined a 2.5‐kb full cDNA sequence of NaPi‐IIa, a 499‐bp partial cDNA sequence of NaPi‐IIb and a 268‐bp partial cDNA sequence of NaPiIIc, which showed 89, 81 and 85% homology with the human isomer sequences, respectively. Tissue distribution analyses showed that NaPi‐IIa mRNA was expressed only in kidney, NaPi‐IIb mRNA only in lung and NaPi‐IIc mRNA in intestine, kidney, lung, liver and heart. The NaPi‐IIc mRNA level in the gut was quantified with real time RT‐PCR (SmartCycler using the SYBR GreenI detection kit), showing that NaPi‐IIc mRNA was low in the duodenum, highly abundant in the proximal jejunum and was present in a decreasing gradient (P<0.05) in the distal jejunum, ileum, cecum and colon. Pigs have a different expression pattern for the NaPi‐II isomers in the viscera with Pi transported across the gut apical membrane by NaPi‐IIc compared with rodents and humans.
Conservé avec la notice de tri, où il sert de preuve aux étiquettes ci-dessus.
La notice
- Revue
- The FASEB Journal
- Thématique
- Parathyroid Disorders and Treatments
- Domaine
- Medicine
- Établissements canadiens
- University of Guelph
- Organismes subventionnaires
- —
- Mots-clés
- IleumMessenger RNACecumJejunumComplementary DNAMolecular biologyBiologyCotransporterEndocrinologyInternal medicineChemistryBiochemistrySodiumGeneMedicine
- Résumé présent dans OpenAlex
- oui