Visceral distribution of the type II sodium‐dependent phosphate cotransporter (NaPi‐II) isomer mRNA and the expression of NaPi‐IIc mRNA along the intestinal longitudinal axis in the post‐weaned pig
Bibliographic record
Abstract
The solute carrier family SLC34 includes the sodium‐phosphate cotransporter (NaPi) type IIa, b, c isomers in rodents and humans. The objective of this study was to identify the NaPi‐II isomers in porcine viscera and to quantify the NaPi‐II isomer mRNA in the gut of the weaned Yorkshire pig. We determined a 2.5‐kb full cDNA sequence of NaPi‐IIa, a 499‐bp partial cDNA sequence of NaPi‐IIb and a 268‐bp partial cDNA sequence of NaPiIIc, which showed 89, 81 and 85% homology with the human isomer sequences, respectively. Tissue distribution analyses showed that NaPi‐IIa mRNA was expressed only in kidney, NaPi‐IIb mRNA only in lung and NaPi‐IIc mRNA in intestine, kidney, lung, liver and heart. The NaPi‐IIc mRNA level in the gut was quantified with real time RT‐PCR (SmartCycler using the SYBR GreenI detection kit), showing that NaPi‐IIc mRNA was low in the duodenum, highly abundant in the proximal jejunum and was present in a decreasing gradient (P<0.05) in the distal jejunum, ileum, cecum and colon. Pigs have a different expression pattern for the NaPi‐II isomers in the viscera with Pi transported across the gut apical membrane by NaPi‐IIc compared with rodents and humans.
Stored with the screening record, where it is evidence for the labels above.
How this classification was reachedexpand
The three-model screen
all 5,600 screened works →All three models called this out of scope.
Molecular physiology of phosphate cotransporter expression in pigs.
It measures phosphate transporter expression in pigs, not research practice.
Biomedical localization of phosphate cotransporters; physiology, not metaresearch.
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".