Fibrin(ogen) stimulates breast, ovarian, and prostate cancer cell line invasiveness on the chick chorioallantoic membrane
Notice bibliographique
Résumé
1821 The importance of hemostasis in tumor development is increasingly being recognized. Many tumor cells express procoagulant activity consistent with a putative role for coagulation in tumor cell migration, invasion, and metastasis. To explore and observe the affects of coagulation components, particularly fibrin(ogen), on tumor growth and invasion we utilized the chicken chorioallantoic membrane (CAM) model for the propagation of cancer cell lines grown in human plasma and fibrinogen clots. Cell lines of human breast cancer MCF-7, MDAMB231; ovarian cancer 2008, SKOV3; and prostate cancer PC3 were placed onto 9d CAMs (1x10 6 cells) in culture medium (CM), human fibrinogen clot (HFC), or human plasma clot (HPC) and incubated until 11d or 14d. CAMs were fixed in formalin; paraffin embedded and cross sections were prepared. Alterations in the CAM were monitored at 24-hour intervals with light microscopy and digital imaging. Cancer cell invasion into the CAM, angiogenesis, inflammation, and epithelial proliferation were evaluated and quantified on a + to 4+ basis from hematoxylin, eosin stained and immunohistochemically treated sections. Cancer cell colonies formed on all of the CAMs by 24h. In section at 48h cancer cells were observed within the parenchyma of the CAM, associated with damage to or removal of the epithelial layer. Cancer cell invasion increased when cells were grown in HPC (3+) compared to HFC (2+) and cells cultured in HFC displayed a higher level of invasion than CM (+/−) cells. There was limited evidence of angiogenesis, and this was similar among the growth conditions, but epithelial proliferation and inflammation demonstrated a similar trend to invasiveness. Breast cancer cell lines grown in HPC showed the greatest increase in invasive potential (3+), and as well were associated with the most enhanced angiogenesis, epithelial proliferation and inflammation compared to other growth conditions. Ovarian cancer cells displayed an intermediate response to culture in HPC (2+), and prostate cancer cells were the least responsive (+). A correlation between cancer cell invasiveness in the presence of HPC and to a lesser extent in HFC suggests a significant relationship among coagulation factors and metastasis. The increased potential of plasma clot compared to fibrinogen clot is consistent with the involvement of other coagulation components in tumor growth and metastasis. It is possible that a clot environment induces a more aggressive phenotype stimulating the production of matrix metalloproteinases and growth factors. The elucidation of the interplay of other coagulation factors, thrombin or tissue factor, and the distinct mechanisms of the interactions with tumor invasion and metastasis may impact cancer treatment.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».