Evidence of autophagy activity in stage V seminiferous epithelium
Bibliographic record
Abstract
Autophagy involves the breakdown of cytosolic components in conjunction with lysosomes. In this study, we test the prediction that tubulobulbar complexes (TBCs) formed by Sertoli cells in rat testes are associated with autophagy. TBCs are cytoskeleton‐related structures that are formed in association with intercellular junctions in the seminiferous epithelium (SE). TBCs are most defined at apical junctions between Sertoli cells and spermatids, although they also are found at basal junctions between adjacent Sertoli cells. TBCs contain a tubular region surrounded by a cuff of actin and a distal region that is associated with cisternae of endoplasmic reticulum. Mounting evidence suggests that Sertoli cells internalize junction proteins via the budding off of double‐membrane vesicles from TBCs followed by fusion with lysosomes resulting in degradation or recycling of junction proteins to newly forming junctions. We used immunological probes to explore the potential of autophagy activity in the SE. Immunostaining of the epithelium suggests that autophagy marker LC3A is not present at apical TBCs in rat testes as predicted but rather, is present elsewhere in the cells, particularly during Stage V of spermatogenesis. Our results indicate that autophagy is not associated apical junction disassembly in Sertoli cells. Grant Funding Source : NSERC
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".