Biofilm forming ability and exoproteomic analysis of Listeria monocytogenes
Bibliographic record
Abstract
The eradication from the food industry environment of the foodborne pathogenic bacterium Listeria monocytogenes requires a better understanding of its biofilm state. The susceptibility of biofilms of L. monocytogenes, in pure and in co-culture with Pseudomonas aeruginosa, to commercial sanitizers was determined using the Calgary Biofilm Device (CBD). Biofilms produced at 12 oC were generally less susceptible to sanitizers than at 37 oC. In co-culture biofilms, L. monocytogenes although representing only 1% of the co-culture, was in the same order of magnitude as the one obtained when in pure culture (5.5 log CFU/cm). In addition to the CBD, three other methods to assess biofilm formation (crystal violet assay, epifluorescence microscopy observation and cell enumeration on stainless steel) were compared. A significant correlation was found between methods using stainless steel, evidence that data is dependent of the surface material. These results also allowed the selection of two good biofilm producing strains to be used for exoproteome investigation. For both strains, 16 identified exoproteins were more abundantly detected in the biofilm exoproteomes compared to the planktonic counterparts. One of these proteins was the putative cell wall binding protein, Lmo2504. The construction of a deletion mutant on its coding gene, confirmed the positive role of this protein in the biofilm forming ability of the wild type strain. This is the first report on the role of the exoproteome in biofilm formation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".