Decreasing postnatal skeletal muscle protein synthetic activity is associated with a reduction in the relative expression of phosphorylated eEF2 in fed pigs
Bibliographic record
Abstract
This study was conducted to investigate the association between developmental changes of fractional protein synthesis rates (FSR) and the expression of eukaryotic protein synthesis elongation factor 2 (eEF2) in the skeletal muscle of fed pigs. Thirty‐six purebred Yorkshire gilts were used at the ages of d 1, 4, 6, 12 and 20 (suckling) as well as d 28 (1 wk post‐weaning). Piglets were given intraperitoneally a flooding dose of Phe containing L‐[ring‐ 2 H 5 ]Phe in saline. Plasma and loin muscle samples at 30 min post‐injection were collected for the determination of tracer Phe enrichment by GC‐MS. Total and phosphorylated (Thr56) forms of eEF2 were examined by Western blot. The FSR of skeletal muscle decreased linearly (P<0.05) from d 1 (20.8 %/d) to day 28 (5.3 %/d). Both total and the phosphorylated forms of eEF2 abundances were linearly decreased (P<0.05) and were correlated with FSR (P<0.05; r= 0.48 and 0.53) at the ages of d 1 to 28. However, the ratio of the phosphorylated eEF to total eEF abundance was linearly increased (P<0.05). These results indicate that the decreasing FSR of skeletal muscle are associated with a reduced relative expression of the phosphorylated eEF2 in fed pigs during the postnatal growth. Supported by NSERC of Canada.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".