Cytological Evidence of Cell-Nuclear Genome Size of a New Ultra-Small Unicellular Freshwater Green Alga, “<i>Medakamo hakoo</i>” strain M-hakoo 311 I. Comparison with <i>Cyanidioschyzon merolae</i> and <i>Ostreococcus tauri</i>
Bibliographic record
Abstract
Ultra-small unicellular algae provide information on the basic cellular mechanisms and essential genes that support the lives of photosynthetic eukaryotes, including higher plants. We have discovered the smallest free-living photosynthetic picoeukaryote to date, a green alga, “Medakamo hakoo” (provisional name) strain M-hakoo 311, which was isolated from freshwater. Based on its pigment composition, M. hakoo belongs to the Chlorophyta. Its cell size and nuclear genome size were compared with those of the primitive red alga Cyanidioschyzon merolae, which lives in freshwater, and with the green alga Ostreococcus tauri, which lives in seawater. The minor and major axes of M. hakoo, C. merolae and O. tauri in the G1 phase were 0.73 and 0.98 μm, 1.2 and 2.37 μm, and 0.76 and 0.96 μm, respectively. The cell size of M. hakoo was thus very similar to that of O. tauri. The nuclear genome sizes of the three algae in the G1 phase were examined using the video-intensified photon-counting system (VIMPCS). The nuclear genome size of C. merolae has previously been determined as 16.5 Mbp by sequencing. Using that value as a standard, the genome sizes of M. hakoo and O. tauri were determined as 9.2 Mbp and 20 Mbp, respectively.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.003 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.005 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.004 | 0.002 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.005 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".