High MUC2 production in goblet cells caused ER stress and susceptibility to apoptosis
Bibliographic record
Abstract
MUC2 produced by goblet cells is the gel forming mucin in mucus that protects the intestinal epithelium against harmful substances and the development of tumors. In this study, we investigated the role of MUC2 in colonic epithelial cell apoptosis, a process essential in preventing tumor formation. A high MUC2‐producing colonic goblet cell line (HT29‐H) and a clone silenced for MUC2 (HT29‐L) by lentivirus‐shRNA were used. Cells were exposed to apoptosis‐inducing agents and apoptosis quantified by caspase 3 and Poly (ADP‐ribose) polymerase cleavage, DNA fragmentation and lactate dehydrogenase (LDH) release. Expression of endoplasmic reticulum (ER) stress markers was evaluated to monitor ER activities as well as expression of genes involved in MUC2 biosynthesis. HT29‐H was found to be highly susceptible to apoptosis as compared to HT29‐L cells. Over expression of MUC2 in HT29‐H cells caused increased expression of ER stress markers and decreased MUC2 folding genes as compared to HT29‐L or parental wild‐type cells. Our findings indicate that MUC2 induces apoptosis in an ER stress‐dependent manner and strongly suggests that it may be a useful therapeutic agent in colorectal carcinomas. Supported by CIHR.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".