Differential regulation of adipocyte and osteoblast differentiation of mesenchymal stem cells by Chemerin and CMKLR1
Bibliographic record
Abstract
Adipocytes and osteoblasts are derived from a common mesenchymal stem cell progenitor within the bone marrow. Previous studies have identified an inverse relationship between adipocyte and osteoblast differentiation of these cells in bone marrow. Work from our laboratory identified chemerin as a novel adipokine that together with its cognate receptor, CMKLR1 plays an indispensable role in regulating adipocyte differentiation of 3T3‐L1 preadipocytes. In the present study we hypothesized that chemerin and its cognate receptor, CMKLR1 is also essential for adipocyte differentiation of bone marrow‐derived mesenchymal stem cells and may simultaneously competitively suppress the alternate osteoblastogenic program. Knockdown of chemerin or CMKLR1 by short hairpin loop RNAs (shRNA) in murine bone marrow primary mesenchymal stem cells abrogated preadipocyte clonal expansion, adipogenic gene expression and lipid accumulation along with the promotion of mineralization and osteoblastogenic gene expression. Thus we conclude that chemerin is essential for bone marrow mesenchymal stem cell adipogenesis and may negatively impact osteoblastogenesis in these cells. Funding: 1. Canadian Institutes of Health Research 2. Nova Scotia Health Research Foundation
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".