Characterization and mutation of soybean MYB transcription factor GmMYBZ2 and its prokaryotic expression.
Bibliographic record
Abstract
Soybean (Glycine max) GmMYBZ2 is one of typical plant R2R3-MYB family genes. In this study, its protein characterization and transcriptional activity were analyzed by DNAMAN and yeast (Saccharomyces cerevisiae) one-hybrid system, respectively. Moreover, the prokaryotic expression vector was constructed and expressed in Escherichia coli as well. Results showed that GmMYBZ2 not only exhibited typical features of the R2R3-MYB transcription factors, but also had a conserved amino acid motif and a predicted zinc finger region at its C-termini. Genomic DNA sequence analysis showed that it contained an intron in the R3 domain which was located at 263~395 bp. In order to investigate the infections of these motifs in GmMYBZ2’s transcriptional activity, the conserved motif and zinc finger region were detected by PCR. The effect of the altered proteins were monitored using yeast one-hybridize, which showed that GmMYBZ2 had transcriptional activation function. And the intron, conserved motif and zinc finger region might repress the transcriptional activity of GmMYBZ2. Moreover, the encoding sequence of GmMYBZ2 gene was inserted into pET30a to construct prokaryotic expression vector pET-ZD2 which was then transformed into E. coli BL21 (DE3) pLysS and Rosetta2 (DE3) pLysS, respectively. Results showed that GmMYBZ2 could be expressed in E. coli Rosetta2 (DE3) which contained complementary rare codons successfully.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".