Gestational effects of maternal folic acid supplementation on DNA methylation in newborn rat pups (827.11)
Bibliographic record
Abstract
Gestational effects of maternal folic acid supplementation on DNA methylation in newborn rat pups Anna Ly, Lisa Ishiguro, Denise Kim, David Im, Sung‐Eun Kim, Kyoung‐Jin Sohn, Young‐In Kim University of Toronto & St. Michael’s Hospital DNA methylation is reprogrammed in utero and maternal folic acid (FA) supplementation has been shown to modulate DNA methylation in the offspring. We determined which gestation period is most sensitive to the effect of maternal FA supplementation on DNA methylation. Pregnant rats were randomized to 5 FA interventions: a control diet throughout pregnancy, FA supplementation at 2.5x the control in 1 st , 2 nd , or 3 rd week gestation only or throughout pregnancy. Pups were killed at birth and blood, brain, liver, kidney and colon processed. Pups from dams fed the control diet throughout pregnancy had significantly lower plasma folate levels compared to the FA supplemented groups (p<0.001). Pups from dams supplemented with FA during the 2 nd or 3 rd week gestation or throughout pregnancy had significantly higher brain folate levels compared to other groups (p<0.001). Pups from dams supplemented with FA throughout pregnancy had significantly higher liver folate levels compared to other groups (p=0.005). Brain global DNA methylation incrementally decreased from early to late gestational FA supplementation and was the lowest in pups from dams supplemented with FA throughout pregnancy (p=0.026). Liver global DNA methylation was lower in pups from dams supplemented with FA throughout pregnancy compared with other groups (p=0.07). No significant difference in folate levels and DNA methylation was observed in the kidney or colon among the 5 groups. Pups from dams supplemented with FA in late gestation or throughout pregnancy had lower Er‐, Gr and Ppar‐ gene expression in the liver compared to other groups (p<0.05). Our data suggest that maternal FA supplementation affects tissue folate levels, DNA methylation and gene expression in an organ and gestation‐specific manner.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".