Serine protease modulation of epithelial barrier function through the tight junction (650.8)
Bibliographic record
Abstract
Inflammatory mediator‐induced disruption of the tight junction may contribute to pathogenesis of IBD through increased intestinal permeability. We have previously found apical addition of the serine proteases trypsin and matriptase to colonic epithelial cell lines increase transepithelial electrical resistance (TER). We aimed to elucidate the role of tight junction (TJ) modulation in this phenomenon. Confluent monolayers of SCBN colonic epithelial cells were mounted on Ussing chambers after being transfected with siRNA against occludin and treated with 45 BAU/mL trypsin. Trypsin‐induced changes in TER and permeability to 4 kDa FITC‐dextran were monitored. siRNA reduced occludin levels by 90% (Western blot) and reduced the trypsin‐induced increase in TER to 29.8 ± 6.0% (n=4 p<0.05) of control response. However, no changes in trafficking of any TJ protein were observed by Triton X‐100 fractionation and Western blotting after trypsin treatment. In a calcium‐switch assay performed in Ussing chambers, trypsin did not induce faster recovery or have an affect on TER until 30 minutes post‐recovery of the monolayer. In conclusion, we have shown that occludin is essential in the serine protease‐mediated increase in TER but this is not through trafficking to the TJ, and that trypsin does not induce the formation of the tight junction. Grant Funding Source : Supported by Canadian Institutes of Health Research
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".