Laminin‐332 deficient mice present defects during tooth enamel development (LB547)
Bibliographic record
Abstract
The epithelial ameloblasts are separated from the maturing enamel surface by an atypical basal lamina (BL) that is enriched in laminin (Lm)‐332. This heterotrimeric protein (α3, ß3 and γ2 chains) provides structural integrity to BLs and exerts effects on various epithelial cell processes including cell adhesion. Mouse models that lack expression of individual Lm‐332 chains die shortly after birth. However, recently a viable mouse model of Lm‐332 deficiency has been developed which expresses a doxycycline‐controllable human Lm γ2 transgene under the cytokeratin 14 promoter on the Lm γ2 knockout background (PLoS One 7(9):e45546). To investigate the role of Lm‐332 in enamel development, we examined the upper and lower maxillae from ~8‐week‐old wt and Lm‐332 deficient mice. At the light microscope level, incisors from Lm‐332 deficient mice showed severe structural changes at the level of the enamel organ. In decalcified samples, the organic matrix of forming enamel was altered and residual matrix was found throughout the maturation stage, suggesting Lm‐332 is required for both formation and mineralization of enamel. CT‐scans and scanning electron microscope analyses showed that Lm‐332 deficient mice exhibited hypomineralized enamel layer and occlusal surface wear in molars. The alterations in enamel formation observed in this mouse model are similar to those in teeth of patient with junctional epidermolysis bullosa due to Lm‐332 mutations. These mice will be an invaluable tool to study the role of Lm‐332 in enamel formation and better understand the role of the atypical BL found at cell‐enamel interfaces. Grant Funding Source : Supported by CIHR, NIH/NHLBI P01HL029594, Shriners of North America
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.003 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.014 | 0.004 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".