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Genotype‐specific <i>IL8RA</i> gene expression in bovine neutrophils in response to <i>Escherichia coli</i> lipopolysaccharide challenge

2008· article· en· W1500555535 on OpenAlexafffundabout
Ivan Leyva-Baca, Gina M. Pighetti, Niel A. Karrow

Bibliographic record

VenueAnimal Genetics · 2008
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicMilk Quality and Mastitis in Dairy Cows
Canadian institutionsUniversity of Guelph
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsBiologyGenotypeLipopolysaccharideSNPEscherichia coliGenePopulationMolecular biologyIn vitroGene expressionGeneticsAndrologySingle-nucleotide polymorphismImmunology

Abstract

fetched live from OpenAlex

Associations between the AC150887.4:c.-1768T>A SNP (rs41255711), which is located in the 5' upstream region of the IL8RA gene (also known as CXCR1), and the estimated breeding values for somatic cell score in the first (P = 0.019) and second (P = 0.035) lactations were previously reported in a population of Canadian Holstein bulls. In the present study, we evaluated the impact of this SNP on the expression of IL8RA by qRT-PCR. Neutrophils were isolated from whole blood samples from a group of cows with genotypes c.-1768AA (n = 4), c.-1768AT (n = 5) and c.-1768TT (n = 5) after the cows had been challenged in vitro with lipopolysaccharide (LPS). This study demonstrated that LPS-induced expression of IL8RA in cows with the c.-1768AA genotype was significantly greater when compared with the c.-1768AT and c.-1768TT genotypes (P < 0.05) before as well as after in vitro LPS challenge.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.693
Threshold uncertainty score0.511

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.039
GPT teacher head0.241
Teacher spread0.202 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations8
Published2008
Admission routes3
Has abstractyes

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