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Zipper‐interacting protein kinase is a key regulator of vascular smooth muscle tone with implications in development of hypertension (676.18)

2014· article· en· W1511928168 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueThe FASEB Journal · 2014
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMedicinal Plants and Bioactive Compounds
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsVascular smooth muscleRho-associated protein kinaseMyocyteProtein kinase AMuscle toneInternal medicineBiologyRegulatorKinaseMedicineCell biologyNeuroscienceSmooth muscle

Abstract

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Essential hypertension is linked to increased contractile tone in the resistance vasculature and affects ~25% of adults, increasing their risk for more severe cardiovascular disease. Recently, zipper‐interacting kinase (ZIPK) was linked to essential hypertension in animal models of the disease. We possess a novel inhibitor of ZIPK that lacks off‐target effects against other contractile kinases and is uniquely situated to investigate the role of ZIPK in normal and pathological vascular smooth muscle function. Hypothesis: ZIPK contributes to the Ca2+ sensitization of vascular smooth muscle, which is in turn accentuated in hypertensive disease states. We used isolated ex vivo vessels with pressure myography, along with the ZIPK inhibitor HS38, to investigate cerebral vessels isolated from human biopsies and spontaneously hypertensive rats (SHR). ZIPK was expressed in human cerebral vessels and contributes to the myogenic response to pressure. Treatment with HS38 resulted in a ~60% reduction in the magnitude of myogenic contractions over the 60‐120mmHg pressure range. Moreover, when early‐stage SHR were compared, we found the myogenic response was enhanced with an increase in the contribution of ZIPK. Based on these findings, we conclude that ZIPK is a critical factor in the development of essential hypertension, and represents a unique and viable, therapeutic target in humans.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.049
Threshold uncertainty score0.215

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.254
Teacher spread0.231 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it