Development of Functional Markers for a Lipoxygenase Gene <i>TaLox‐B1</i> on Chromosome 4BS in Common Wheat
Bibliographic record
Abstract
ABSTRACT Lipoxygenase (LOX) activity in grain influences the color and processing quality of wheat (Triticum spp.)‐based products. Characterization of LOX genes and development of functional markers are of importance for marker‐assisted selection in common wheat (Triticum aestivum L.) breeding. In the present study, the full‐length genomic DNA (gDNA) sequence of a LOX gene (designated TaLox‐B1) located on chromosome 4BS was characterized by in silico cloning and experimental validation. Two complementary dominant sequence tagged site (STS) markers, LOX16 and LOX18, were developed based on the single nucleotide polymorphism (SNP) of two alleles at the TaLox‐B1 locus, amplifying 489‐ and 791‐bp fragments in cultivars with higher and lower LOX activities, respectively. The two markers were mapped on chromosome 4BS using a doubled haploid (DH) population derived from Zhongyou 9507 × CA9632, a set of Chinese Spring nullisomic–tetrasomic lines, ditelosomic line 4BS, and Langdon (LDN) 4D(4B) chromosome substitution line. Quantitative trait loci (QTL) analysis indicated that TaLox‐B1 co‐segregated with the two functional markers and was closely linked to simple sequence repeat (SSR) locus Xgwm251 on chromosome 4BS with a genetic distance of 1.8 cM. LOX16 and LOX18 were validated on 198 Chinese wheat cultivars and advanced lines and showed highly significant (p < 0.01) associations with LOX activity. These results suggested that LOX16 and LOX18, co‐segregating with TaLox‐B1, could be used for the improvement of color attributes of noodles and other wheat‐based products in wheat breeding programs.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".