Bt W015-1, A <i>Bacillus thuringiensis</i> Strain from Diseased Larva of Silkworm with Highly Larvacidal Toxicity against Lepidopteran Insects
Bibliographic record
Abstract
In 2005, Dr. X. J. Fang’s group from Hainan Institute of Tropical Agricultural Resources (HITAR) isolated a Bacillus thuringiensis isolate (isolate No:20050509W015) from the mid-gut of diseased and dead larva of silkworm ( Bombyx mori ) that were collected from the sericulture farmers of the region of Hangjiahu (Hangzhou, Jiaxing and Huzhou) in Zhejiang province of China. Bt W015-1 was found to produce crystalline protein toxins with high larvicidal effects against Lepedopterans. Scanning electron microscope observation showed that the strain can produce high-yielding bipyramid crystals during sporulation. SDS-PAGE analysis showed that the protein is about 130 kDa in. Furthermore, bioassays conducted on the diamondback moth demonstrated that the strain is highly toxic. The plasmid profile of the Bt W015-1 exhibited in most part no difference in size and band spectrum from model strain HD73 of Btt, but showed striking difference from the HD1 strain of Btt and Bti. The cry genotype is significantly different in cry1Ac restriction cutting sites from the standard strain HD73 of Btt. The cloned gene cry1Ac22 gene (Accession number EU282379), which encodes a protein of 1 178 amino acid residues, and it was ligated into E. coli M15, can express 130 kDa proteins that show high insecticidal activities against lepidopteran diamondback moth ( Plutella xylostella ).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".