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Record W1546762202 · doi:10.1002/9780470054581.eib012

Affinity Fusions: Gene Expression

2009· other· en· W1546762202 on OpenAlexfundno aff
S. Gräslund, M. Hammarstrom

Bibliographic record

VenueEncyclopedia of Industrial Biotechnology · 2009
Typeother
Languageen
FieldMedicine
TopicMonoclonal and Polyclonal Antibodies Research
Canadian institutionsnot available
FundersCanadian Institutes of Health ResearchKnut och Alice Wallenbergs StiftelseGenome Canada
KeywordsComputational biologyProteomeRecombinant DNAFusion proteinAffinity chromatographyProteomicsFolding (DSP implementation)Protein tagBiochemistryBiologyChemistryComputer scienceGeneEngineeringEnzyme

Abstract

fetched live from OpenAlex

Abstract In response to the rapidly growing field of proteomics, the use of recombinant proteins has increased greatly in recent years. Since proteins have very diverse physiological properties, they are not naturally adjusted to generic methods. Affinity tags, providing a common handle for all kinds of proteins, have consequently become indispensable tools for structural and functional analysis of whole proteomes. Although originally developed to facilitate the detection and purification of recombinant proteins, it has become clear in recent years that affinity tags can also have positive impact on the yield, solubility, in vivo half‐life, and even the folding of their fusion partners. However, no single affinity tag is optimal with respect to all these parameters. Each has its strengths and weaknesses and therefore a suitable tag must be chosen for each application. An alternative would be the use of combinatorial tags and thus combining several desired properties.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity, Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.098
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0040.002
Insufficient payload (model declined to judge)0.0050.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.035
GPT teacher head0.297
Teacher spread0.263 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designNot applicable
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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