Bacterial Acyl Carrier Protein Interacts with the Alarmone SpoT and the Adaptive Response Protein AidB: Roles for Acyl Carrier Protein Beyond Lipid Metabolism
Bibliographic record
Abstract
Acyl carrier protein (ACP) plays an essential role in the synthesis of bacterial lipids, and is an attractive target for the development of new antibiotic classes. ACP also forms numerous interactions with proteins involved in other cellular processes that have yet to be fully identified or characterized. Using a C‐terminal Tandem Affinity Purification (TAP)‐tagged ACP we identified two ACP binding partners not directly involved in lipid metabolism, AidB and SpoT, that both exhibit increased interaction with ACP in stationary phase. The increase in binding of SpoT, a synthetase and hydrolase of the alarmone guanidine ‐ 5' (tri)diphosphate ‐3'diphosphate, to ACP appears to be due to increased affinity between the two proteins as cells enter stationary phase. In contrast, the increased interaction with AidB, an adaptive response protein, results from elevation in the level of AidB as cells enter stationary phase. AidB levels are also increased upon exposure of cells to alkylating agents. AidB binds DNA directly and we hypothesize that the positively charged DNA binding region of AidB also binds to the negatively charged ACP. AidB binding to ACP may have important functional consequences in DNA repair. To test this, ACP‐AidB protein‐protein interaction domains are being mapped, and we are determining if AidB‐ACP interactions regulate the ability of the cell to respond to DNA damaging agents. (Supported by NSERC)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".