48 PREGNANCY AND FETAL CHARACTERISTICS AFTER TRANSFER OF VITRIFIED IN VIVO AND CLONED BOVINE EMBRYOS
Bibliographic record
Abstract
This study was conducted to examine pregnancy progression and fetal characteristics following transfer of vitrified bovine nuclear transfer (NT) vs. in vivo-derived (Vivo) embryos. Nuclear transfer was conducted using skin fibroblast cells derived from cultured ear explants taken from an elite Holstein-Friesian dairy cow. Expanding and hatching blastocysts on Day 7 were vitrified using liquid nitrogen surface vitrification (Shen et al. 2004 Reprod. Fertil. Dev. 16, 158). Day 7 in vivo embryos, produced using standard superovulation procedures applied to Holstein-Friesian heifers (n = 6), were vitrified in the same way. Following warming, embryos were transferred to synchronized recipients (2 per recipient; NT: n = 81 recipients; Vivo: n = 20 recipients). Pregnancies were monitored by ultrasound scanning on Days 25, 45, and 75, and a sample of animals was slaughtered at each time point to recover the fetus/placenta for further analyses. On Day 25, the entire conceptus was weighed together; on Day 45, the fetus and placenta were weighed separately; on Day 75, the fetus was dissected and the major organs were weighed. Significantly more animals remained pregnant after transfer of in vivo-derived embryos than NT embryos at all time points: Day 25 (95.0 vs. 61.7%, P < 0.01), Day 45 (93.3 vs. 39.7%, P < 0.001), and Day 75 (90.9 vs. 19.8%, P < 0.001). There was no significant difference (P = 0.10) in the weight of the conceptus on Day 25 from NT transfers (1.14 ± 0.23 g, n = 8) vs. Vivo transfers (0.75 ± 0.19 g, n = 8). On Day 45, there was no significant difference in the weight of either fetus (P = 0.393) or membranes (P = 0.167) between NT embryos (fetus: 2.76 ± 0.40 g, n = 12; membranes 59.0 ± 10.0 g, n = 11) or in vivo-derived embryos (fetus: 2.60 ± 0.15 g, n = 6; membranes 41.8 ± 5.2 g, n = 4). However, on Day 75, the weights of the fetus and several of the major organs were heavier from NT embryos (Table 1). These data suggest that the large calf syndrome is manifested after Day 45. Table 1. Fetal characteristics on Day 75 of gestation following transfer of vitrified in vivo-derived vs. NT blastocysts This work was supported by the U.S. Department of Agriculture.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".