Role of Intracellular Ca <sup>2+</sup> in EDRF Release in Rat Aorta
Bibliographic record
Abstract
In rings of rat aorta, cyclopiazonic acid (CPA), a selective inhibitor of the internal membrane Ca(2+)-pump ATPase, gradually initiated a concentration-dependent contraction which was much less in endothelium-intact than in endothelium-denuded rings. In phenylephrine-precontracted rings with intact endothelium, CPA, like acetylcholine, produced endothelium-dependent relaxations in a concentration-dependent manner. These were nearly abolished by methylene blue (MB) or NG-nitro-L-arginine methylester (L-NAME). This inhibitory effect of L-NAME was reversed by L-arginine but not by D-arginine, indicating that CPA induced a release of endothelium-derived relaxing factor (EDRF) from endothelial cells leading to smooth muscle relaxation. Concentration-dependent relaxations induced by sodium nitroprusside, which is thought to act by releasing nitric oxide, were not inhibited by L-NAME, but were inhibited similarly by MB in endothelium-intact and -denuded rings. These results indicate that CPA causes EDRF-release from endothelial cells and support the recent hypothesis that release of intracellular Ca from stores is the initiating factor in EDRF release, possibly allowing ongoing Ca entry to sustain release.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".