Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
Bibliographic record
Abstract
Rab proteins are key regulators of membrane transport in eukaryotes. Recent evidence from different species supports the notion that some Rab proteins are crucial for cytokinesis, a pivotal procedure for successful cell division. As a family of monomeric small GTPases of the Ras superfamily, the function of Rab proteins is modulated by guanine nucleotide binding and hydrolysis. To investigate the function of Rab proteins, creating dominant negative or constitutively active mutant forms of a Rab protein is a widely used approach. To study cytokinesis in plant cells, using fluorescent dye to highlight the cell shape and the nuclei, and to monitor the formation of the newly formed cell plate in mitotic cells, is easy and useful. In this chapter, we describe detailed methods for (1) generating transgenic plants expressing dominant negative or constitutively active form of RAB-A1c; (2) fluorescent staining of cell shape, cell wall, and nuclei of mitotic root tip cells; (3) fluorescent staining of newly formed cell plate; and (4) detecting fluorescent signals using Confocal Laser Scanning Microscopy in the genetic model plant species Arabidopsis thaliana.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".