Intracellular pH Measurement with Fluorescent Dye in Canine Basilar Arteries
Why this work is in the frame
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Bibliographic record
Abstract
The intracellular pH (pH<sub>i</sub>) of basilar artery rings was determined with 2’,7’-bis-(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF) by measuring the ratio of emitted (540 nm) fluorescence intensities (FI) at excitation wavelengths of 500 and 440 nm. There was a dye loss from the rings in 90 min (39.3 ± 3.6%; p < 0.001). We found that the ratio of fluorescence intensities does not adequately correct for dye loss; hence, we derived a method to correct for dye loss during pHi determinations. Calibration curves of the ratio versus pH, were constructed for the artery rings. The slope and intercept of the calibration curves depended on FI<sub>440</sub>. Linear regression lines for the slope and intercept versus FI440 were: slope = 0.00343 × FI<sub>440</sub> + 0.32484 (r – 0.96; p < 0.0001) and intercept = –0.02048 × FI<sub>440</sub> – 0.80123 (r = –0.97; p < 0.0001). In solutions with different pH and different concentrations of free BCECF, the slope of the ratio versus pH of the solution was steeper at high concentrations of BCECF. Thus, pH was calculated from a calibration curve in which the slope and intercept were determined from FI440 with the above formula. The corrected pH<sub>i</sub> was 7.37 ± 0.05 (n = 25) at pH<sub>o</sub> 7.4 and 37 °C.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it