The regulation of the cell surface proteome by AMP‐activated protein kinase (604.5)
Bibliographic record
Abstract
The many proteins at the cell surface (collectively, the cell surface proteome) control numerous cellular functions. The amount of each protein at the cell surface is dynamically controlled by endocytosis and recycling. AMP‐activated protein kinase (AMPK) is an important metabolic regulator that responds to energetic stress, such as increased cellular AMP:ATP and certain hormones. Studies examining a small number of cell surface proteins suggest that AMPK may have an important role in regulating endomembrane traffic. How AMPK may more broadly regulate the cell surface proteome is not known. We have refined a method to isolate the cell surface proteome from cultured cells, involving biotinylation of surface‐exposed proteins in intact cells and purification of biotinylated proteins, which we coupled to quantitative mass spectrometry to identify novel AMPK‐regulated membrane traffic phenomena. We find that activation of AMPK results in a decrease in the cell surface abundance of the β1‐integrin, revealing a mechanism by which cellular metabolism may regulate cell adhesion and migration. Our results provide novel insight into the regulation of the cell surface proteome by metabolic stress signaling.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".