The pioneer factor PBX1 is a novel driver of metastatic progression in ERα-positive breast cancer
Bibliographic record
Abstract
// Luca Magnani 1 , Darren K. Patten 1 , Van T.M. Nguyen 1 , Sung-Pil Hong 1 , Jennifer H. Steel 1 , Naina Patel 1 , Ylenia Lombardo 1 , Monica Faronato 1 , Ana R. Gomes 1 , Laura Woodley 1 , Karen Page 2 , David Guttery 2 , Lindsay Primrose 2 , Daniel Fernandez Garcia 2 , Jacqui Shaw 2 , Patrizia Viola 3 , Andrew Green 4 , Christopher Nolan 4 , Ian O. Ellis 4 , Emad A. Rakha 4 , Sami Shousha 1 , Eric W.-F. Lam 1 , Balázs Győrffy 5 , Mathieu Lupien 6, 7 , R. Charles Coombes 1 1 Department of Surgery and Cancer, Imperial College London, London, UK 2 Department of Cancer Studies, University of Leicester, Leicester, UK 3 Laboratory of Medicine, Histopathology Department, Royal Brompton Hospital, London, UK 4 Division of Cancer and Stem Cells, School of Medicine, University of Nottingham, Budapest, HU 5 MTA TTK Lendület Cancer Biomarker Research Group; 2nd Department of Pediatrics, Semmelweis University and MTA-SE Pediatrics and Nephrology Research Group, Budapest, HU 6 The Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada 7 Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada Correspondence to: Luca Magnani, e-mail: l.magnani@imperial.ac.uk Keywords: breast cancer, estrogen receptor, drug resistance, metastasis, pioneer factors Received: February 18, 2015 Accepted: June 02, 2015 Published: June 15, 2015 ABSTRACT Over 30% of ERα breast cancer patients develop relapses and progress to metastatic disease despite treatment with endocrine therapies. The pioneer factor PBX1 translates epigenetic cues and mediates estrogen induced ERα binding. Here we demonstrate that PBX1 plays a central role in regulating the ERα transcriptional response to epidermal growth factor (EGF) signaling. PBX1 regulates a subset of EGF-ERα genes highly expressed in aggressive breast tumours. Retrospective stratification of luminal patients using PBX1 protein levels in primary cancer further demonstrates that elevated PBX1 protein levels correlate with earlier metastatic progression. In agreement, PBX1 protein levels are significantly upregulated during metastatic progression in ERα-positive breast cancer patients. Finally we reveal that PBX1 upregulation in aggressive tumours is partly mediated by genomic amplification of the PBX1 locus. Correspondingly, ERα-positive breast cancer patients carrying PBX1 amplification are characterized by poor survival. Notably, we demonstrate that PBX1 amplification can be identified in tumor derived-circulating free DNA of ERα-positive metastatic patients. Metastatic patients with PBX1 amplification are also characterized by shorter relapse-free survival. Our data identifies PBX1 amplification as a functional hallmark of aggressive ERα-positive breast cancers. Mechanistically, PBX1 amplification impinges on several critical pathways associated with aggressive ERα-positive breast cancer.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".