<i>Allium cepa</i>L. and Quercetin Inhibit RANKL/<i>Porphyromonas gingivalis</i>LPS-Induced Osteoclastogenesis by Downregulating NF-<i>κ</i>B Signaling Pathway
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Post-publication record
- Nature
- Retraction
- Reason
- Duplication of/in Image;Error in Image;Investigation by Journal/Publisher;Investigation by Third Party;Original Data and/or Images not Provided and/or not Available;
- Date
- 8/14/2019 0:00
- Flagged by OpenAlex?
- Yes
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Abstract
Objectives. We evaluated the in vitro modulatory effects of Allium cepa L. extract (AcE) and quercetin (Qt) on osteoclastogenesis under inflammatory conditions (LPS-induced). Methods. RAW 264.7 cells were differentiated with 30 ng/mL of RANKL, costimulated with PgLPS (1 µg/mL), and treated with AcE (50-1000 µg/mL) or Qt (1.25, 2.5, or 5 µM). Cell viability was determined by alamarBlue and protein assays. Nuclei morphology was analysed by DAPI staining. TRAP assays were performed as follows: p-nitrophenyl phosphate was used to determine the acid phosphatase activity of the osteoclasts and TRAP staining was used to evaluate the number and size of TRAP-positive multinucleated osteoclast cells. Von Kossa staining was used to measure osteoclast resorptive activity. Cytokine levels were measured on osteoclast precursor cell culture supernatants. Using western blot analysis, p-IκBα and IκBα degradation, inhibitor of NF-kappaB, were evaluated. Results. Both AcE and Qt did not affect cell viability and significantly reduced osteoclastogenesis compared to control. We observed lower production of IL-6 and IL-1α and an increased production of IL-3 and IL-4. AcE and Qt downregulated NF-κB pathway. Conclusion. AcE and Qt may be inhibitors of osteoclastogenesis under inflammatory conditions (LPS-induced) via attenuation of RANKL/PgLPS-induced NF-κB activation.
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The record
- Venue
- Evidence-based Complementary and Alternative Medicine
- Topic
- Bone Metabolism and Diseases
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- University of Toronto
- Funders
- Fundação de Amparo à Pesquisa do Estado da BahiaConselho Nacional de Desenvolvimento Científico e TecnológicoUniversity of TorontoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Keywords
- Porphyromonas gingivalisRANKLNF-κBChemistrySignal transductionMedicinePeriodontitisReceptorBiochemistryInternal medicine
- Has abstract in OpenAlex
- yes