Engineering <i>Escherichia coli</i> for D-Ribose Production from Glucose-Xylose Mixtures
Bibliographic record
Abstract
D-ribose is a commercially important functional sugar used as a nutritional supplement and as a starting compound in the synthesis of antiviral drugs. In this study, we report engineered Escherichia coli mutants that can produce D-ribose from glucose and xylose. Two endogenous haloacid dehalogenase-like (HAD) phosphatases from E. coli, HAD12 and HAD13, encoded by the genes ybiV and yidA, respectively, were expressed in E. coli wild type and the glucose-xylose co-utilizing mutant LMSE2. All the mutants constructed in this study produced D-ribose. The mutant RB-006 (LMSE2 expressing ybiV) showed the highest D-ribose titer of 1.16 g/L from 5 g/L each of glucose and xylose. Additionally, using xylose feeding, D-ribose titer was improved to 3.36 g/L. Xylulose and acetate were formed as the major byproducts in the fed-batch study. This study represents the first example of engineered E. coli for production of D-ribose. This study also demonstrates reengineering of a glucose-xylose co-utilizing mutant of E. coli for production of a valuable chemical.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".