Engineering <i>Escherichia coli</i> for D-Ribose Production from Glucose-Xylose Mixtures
Why this work is in the frame
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Bibliographic record
Abstract
D-ribose is a commercially important functional sugar used as a nutritional supplement and as a starting compound in the synthesis of antiviral drugs. In this study, we report engineered Escherichia coli mutants that can produce D-ribose from glucose and xylose. Two endogenous haloacid dehalogenase-like (HAD) phosphatases from E. coli, HAD12 and HAD13, encoded by the genes ybiV and yidA, respectively, were expressed in E. coli wild type and the glucose-xylose co-utilizing mutant LMSE2. All the mutants constructed in this study produced D-ribose. The mutant RB-006 (LMSE2 expressing ybiV) showed the highest D-ribose titer of 1.16 g/L from 5 g/L each of glucose and xylose. Additionally, using xylose feeding, D-ribose titer was improved to 3.36 g/L. Xylulose and acetate were formed as the major byproducts in the fed-batch study. This study represents the first example of engineered E. coli for production of D-ribose. This study also demonstrates reengineering of a glucose-xylose co-utilizing mutant of E. coli for production of a valuable chemical.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it