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Endurance Exercise and Systemic Mitochondrial Rejuvenescence: Run for Your Life!

2010· article· en· W166723759 on OpenAlexafffund
Adeel Safdar, Jonathan P. Little, Bart P. Hettinga, Gregory C. Kujoth, Tomas A. Prolla, Mark A. Tarnopolsky

Bibliographic record

VenueThe FASEB Journal · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMitochondrial Function and Pathology
Canadian institutionsMcMaster University
FundersCanadian Institutes of Health Research
KeywordsMitochondrial biogenesisTFAMMitochondrial DNABiologySkeletal muscleMitochondrionEndurance trainingMitochondrial myopathyPremature agingInternal medicineMitochondrial diseaseEndocrinologyMedicineGeneticsGene

Abstract

fetched live from OpenAlex

A causal role for mitochondrial DNA (mtDNA) mutagenesis in mammalian aging is supported by recent studies demonstrating that the polymerase gamma (PolG) mutator mouse, harbouring a proofreading‐deficient copy of PolG, exhibits an accelerated aging phenotype, systemic mitochondrial dysfunction, multisystem failure, and reduced lifespan. Studies in primary cells from mitochondrial myopathy patients indicate that inducing mitochondrial biogenesis via over‐expression of PGC‐1α has therapeutic potential. We aimed to delineate if endurance exercise‐mediated induction of PGC‐1α metabolic network can prevent premature aging and systemic decline in PolG mice. At 3‐mo, 36 PolG mice (♀ = ♂) were randomly assigned to a sedentary (SED) or forced‐endurance training (END; 15m/min for 45 min, 3x/week for 5 months) group. In skeletal muscle, END increased nuclear abundance of PGC‐1α (76%) while concomitantly decreasing RIP140 (a negative regulator of PGC‐1α; 33%) nuclear content (P<0.05). END also increased mtDNA copy number (2–3 fold), protein content of respiratory chain subunits (CI‐NUFA9, CII‐subunit 70 kDa, C‐III core 2, and COX‐IV; 2–3 fold), and COX activity (22–120%) in skeletal muscle, lungs, heart, brain, and gonads (P<0.05). These molecular adaptations conferred complete phenotypic protection, reduced multi‐organ pathology, and increased lifespan of PolG mice. We conclude that END training promotes PGC‐1α‐mediated systemic mitochondrial oxidative capacity, contributing to the complete rejuvenation of PolG mice. We propose that END training offers a valuable therapeutic intervention for attenuating and/or reversing mitochondrial abnormalities associated with aging. (Funded by CIHR, and Mr. Warren Lammert and family)

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.016

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.251
Teacher spread0.237 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes2
Has abstractyes

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