78 CHRONOLOGICAL EVENTS OF IN VITRO MATURATION IN CAMEL (CAMELUSDROMEDARIES) OOCYTES
Bibliographic record
Abstract
Experiments were conducted to investigate the chronological events and optimum time for in vitro oocyte maturation in the dromedary camel. Follicles measuring 3–12 mm were isolated from ovaries obtained from an abattoir and the oocytes harvested by teasing apart these follicles under a stereo microscope. Pooled oocytes were randomly distributed to 4-well dishes (20–25 per well) (Nunc, Denmark) containing 400 µL of the maturation medium (TCM-199 supplemented with 0.6 mg mL-1 calcium lactate, 0.1 mg mL-1 L-glutamine, 0.8 mg mL-1 sodium bicarbonate, 1.4 mg mL-1 HEPES, 0.25 mg mL-1 pyruvate, 50 µg mL-1 gentamicine, 10 µg mL-1 FSH, 10 µg mL-1 LH, 1 µg mL-1 estradiol and 10% heat-inactivated estrous camel serum) and incubated at 38.5°C under 5% CO2 for 4 to 48 h. After every 4 h (starting from 0 to 48 h), oocytes were denuded by treating them with hyaluronidase (1 mg mL-1) followed by repeated pipetting. Denuded oocytes were mounted on glass slides and fixed in 3 : 1 ethanol : acetic acid for 24 h. Oocytes were stained with 1% aceto-orcein and examined under a phase contrast microscope at 400times. for each experimental group, 3 to 7 replications were made. Based on the visualization of the chromatin, oocytes were categorized as at germinal vesicle (GV), diakinesis (DK), metaphase-I (M-I), anaphase (Ana), metaphase-II (M-II) stage and those with no visible chromatin as NVC. At the start of maturation, 75.4% (43/57) of oocytes were at GV stage; however, none of the oocytes revealed a GV at 28 h of maturation (0/97). At 8 h of maturation 49.3% (34/69) of oocytes were at DK stage, and after 16 h of maturation 50% (49/98) of oocytes were at M-I stage. At 24 h of maturation the maximum number of oocytes were in Ana (24.7%, 21/85) stage. At 44 h the maximum number of oocytes had reached M-II stage (52%, 103/198) whereas, 10.6% (21/198) of the oocytes were at Ana stage. After 48 h the proportion of oocytes with NCV increased to 52.9% (45/85) and the proportion of M-II stage oocytes decreased to 37.6% (32/85). It may be concluded that 40–44 h of in vitro maturation yields the highest proportion of matured (M-II stage) oocytes suitable for further use in assisted reproductive technologies in camel.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".