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Beta‐GPA‐induced changes in mitochondrial biogenesis are associated with a muscle type‐dependent decrease in RIP140

2009· article· en· W169002929 on OpenAlexafffund
Deon B. Williams, Susan A.U. Basaraba, Lindsey Sutherland, Marc R. Bomhof, Brianne Thrush, David J. Dyck, Catherine J. Field, David C. Wright

Bibliographic record

VenueThe FASEB Journal · 2009
Typearticle
Languageen
FieldMedicine
TopicAdipose Tissue and Metabolism
Canadian institutionsUniversity of GuelphUniversity of Alberta
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsMitochondrial biogenesisInternal medicineSkeletal muscleEndocrinologyChemistryCitrate synthaseSoleus muscleActivator (genetics)MitochondrionCell biologyBiologyBiochemistryReceptorEnzyme

Abstract

fetched live from OpenAlex

Beta‐guanidopropionic acid (β‐GPA) is a creatine analogue that leads to reductions in ~P concentrations and increases in markers of mitochondrial content in rodent skeletal muscle. Prior evidence attributes these changes to activation of the 5'AMP pathway; however data suggest RIP140, a negative downstream regulator of PPAR gamma co‐activator 1 alpha (PGC‐1α), is a likely player in the regulation of mitochondrial biogenesis (MB). The aim of this study was to determine if β‐GPA feeding decreases RIP140 protein in skeletal muscle in a fiber type‐specific manner and if it is associated with an increase in mitochondrial content. Male Wistar rats were fed a semi‐purified diet supplemented with (n=12) or without (n=12) 1% β‐GPA for 6 weeks. β‐GPA feeding induced skeletal muscle MB in tricep as shown by increases in CORE1 and COXIV protein and citrate synthase activity. In contrast, no such changes were seen in soleus from β‐GPA‐fed rats. β‐GPA feeding led to a ~75% increase in PGC1α messenger RNA expression in tricep and ~140% increase in soleus. RIP140 protein decreased ~40% in tricep while it was unchanged in the soleus. In summary, β‐GPA feeding led to decreases in RIP140 in rat tricep only, correlating to increases in MB in only this muscle type. This suggests that RIP140 may play a key role in regulating MB downstream of PGC1α and provides a mechanism for fiber type differences in muscle adaptation. Supported by NSERC

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.029
GPT teacher head0.269
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes2
Has abstractyes

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