Studies on Construction of Regeneration System and Genetic Transformation of <i>Puccinellia chinampoensis</i>
Why this work is in the frame
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Bibliographic record
Abstract
The regeneration system was established and the genetic transformation was processed, using the mature seeds of Puccinellia chinampoensis as explants. The results showed that the callus induction rate could reach to 39.75% after 19days of induction with the altered mediumâ… (adding 4 mg/L 2,4-D, 500 mg/L proline and 500 mg/L glutamine) (pH 5.8~6.0). When using the modified medium S (adding 2 mg/L 2,4-D, 1 mg/L ABA, 500 mg/L proline and 600 mg/L caseinhydrolysate) (pH 5.8~6.0) as the subculture medium, we can get much more embryogenic callus. The differentiation rate could reach to 53.73% using the differentiation medium (MS as the basic medium, adding 0.4 mg/L ABA, 0.04 mg/L IAA, 500 mg/L proline and 600 mg/L caseinhydrolysate) (pH 5.8~6.0). On the genetic transformation, the transformation efficiency was best when the infect time lasted 30 min, making using of the Agrobacterium tumefaciens mediated pBI121-GUS transformation when OD 600 reached to 0.8~1.0.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it