Characterization of a novel human CAP2 interacting protein: Neuronal calcium binding protein 1 (Necab1).
Bibliographic record
Abstract
The actin cytoskeleton is implicated in numerous processes such as cellular division, migration, endocytosislexocytosis, and more recently synaptic plasticity. Important players in controlling cytoskeletal rearrangements are the Cyclase Associated Proteins (CAPs) which are thought to link cell signalling to the dynamics of the actin cytoskeleton, via sequestration of monomeric actin subunits. CAPs are conserved throughout evolution and possess conserved functional domains. Previous studies have suggested a link between CAP proteins and vesicle trafficking and this study focussed on characterizing binding partners of human CAPs. Using a two hybrid screen of a human adult brain cDNA library, a novel human CAP interacting protein named Neuronal calcium binding protein 1 (Necab1) has been isolated. Necab1 is a 351 amino acid protein containing a predicted calcium binding EF-hand and three coiled-coil domains. It is highly expressed in human brain, and similar to CAP2, displays high levels of expression within the hippocampus and cerebral cortex of rat brain. In vitro binding and in vivo immunoprecipitations demonstrate that CAP2 interacts with Necab1. (Abstract shortened by UMI.)Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2002 .M68. Source: Masters Abstracts International, Volume: 43-01, page: 0215. Adviser: A. V. Hubberstey. Thesis (M.Sc.)--University of Windsor (Canada), 2003.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".