Extraction and fractionation of polyunsaturated fatty acids from <i>Mortierella</i> sp. using supercritical fluid: experimental and kinetic studies
Why this work is in the frame
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Bibliographic record
Abstract
ABSTRACT The growing interest in various applications of polyunsaturated fatty acids daily dietary requirements and demand has directed much attention on the industrial production of bio‐oil from plant, animal, fungi, or algae sources. This work signifies that the extraction of polyunsaturated fatty acids from fungal biomass of Mortierella sp. 1458 using supercritical CO 2 (SC‐CO 2 ) with subsequent fractionation of various fatty acids present in biomass using co‐solvents was investigated. The use of co‐solvent in supercritical fluid extraction has provided the green system as a substitute for classical solvent extraction at industrial scale. The optimized extraction temperature (62.3 °C), pressure (298.5 bar), and time (88.6 min) on SC‐CO 2 extraction conditions gave maximum recovery up to 66.35% of bio‐oil from dried biomass. Further co‐solvent addition to SC‐CO 2 has improved % recovery up to 97.88%. The bio‐oil obtained by this method was examined for use by characterization with value added chemicals such as omega‐3, omega‐6, and omega‐9 fatty acids. Peleg's equation was used to study the bio‐oil extraction kinetics. The equilibrium yield as well as the time required to reach half of equilibrium yield were also calculated. It showed that for an effective extraction process with high yield, CO 2 density larger than 700 kg/m 3 is needed. © 2014 Curtin University of Technology and John Wiley & Sons, Ltd.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it