Cloning and characterization of the mitochondrial genes <i>Cox II</i> and <i>atpA</i> from ramie (<i>Boehmeria nivea</i> (L.) Gaud.) and their possible role in cytoplasmic male sterility
Bibliographic record
Abstract
Huang, H.-Q., Liu, J.-D., Duan, J.-Q., Liang, X.-N. and Liu, F.-H. 2012. Cloning and characterization of the mitochondrial genes Cox II and atpA from ramie ( Boehmeria nivea (L.)Gaud.) and their possible role in cytoplasmic male sterility. Can. J. Plant Sci. 92: 1295–1304. In plants, cytoplasmic male sterility (CMS) is known to be associated with structural changes and the presence of new chimeric genes in mtDNA. In this study, fragments of the Cox II and atpA genes, cloned from mtDNA of three lines (CMS line, its maintainer and restorer lines) of ramie [Boehmeria nivea (L.) Gaud.], showed homology of 95% for Cox II and 97% for atpA compared with the reference dicotyledons in GenBank. The whole sequences of these two genes were obtained using the DNA walking strategy. Cox II showed no difference among the three lines in sequences of DNA and deduced amino acids. However, atpA from the CMS line obviously differed from that of fertile lines in DNA sequence, amino acid sequence and secondary structure of the predicted protein. Unusually low expression was revealed via RT-PCR for atpA in the CMS line at the budding and late-bloom stages. In conclusion, the variation in DNA sequence and the encoded product, and/or the abnormal expression of atpA in the CMS line, may cause male sterility in ramie.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".