Self orientation of cell and cell‐secreted collagen in a tissue‐engineered cornea grown on microstructured polystyrene
Bibliographic record
Abstract
The corneal stroma is composed of many layers of fibroblasts and collagen that are oriented longitudinally within a layer and the layers display a shift of approximately 60‐degree angle between each other. This type of structure is known to be responsible for the transparency of the cornea. Using the self‐assembly approach to cultured cell sheets, we evaluated how cell‐secreted collagen reaction could be influenced by external physical cues. Microstructured polystyrene samples used for cell culture were prepared by hot embossing, replicated and transferred from a Si master of different grating periods and linewidths to a polystyrene substrate. We observed that cell division occurred only in the longitudinal axis of the gratings instead of the random cell orientation seen in the control. Cells forming the first layer on the substrate aligned themselves in the same longitudinal direction as the gratings. We also observed that after 7 days in culture the cells of the second layer, forming on top of the first one, aligned themselves at an angle of approximately 60 degree from the first one, and so did the cell‐secreted collagen fibres. These results are very promising for the reconstruction of autologous tissue‐engineered cornea since optimization of transparency is of paramount importance for the patients who will benefit from this type of surgery. Financial support is provided by the National Research Council‐Canadian Institutes of Health Research (grant 66473).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".