Genome-wide identification and characterization of heat shock factor genes from pigeonpea (<i>Cajanus cajan</i>)
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Bibliographic record
Abstract
Genome-wide analysis of heat shock factor ( H s f ) g e n e s was ca r r ied out in p i g e on p e a ( Cajanus cajan ) in order to understand their structure and function. A tot a l of 23 Hsfs w e r e p re dict e d using FGENESH a nd l a b e led a s C c Hsf . Out of the 2 3 g e n e s, 14 unique sequences w e r e s e l ec t e d a nd c h a r a c t er i z e d for t h e ir p re sumed str u c tur e s s u c h a s p r ote i n doma in a nd mo t if o r g a ni z a t i on. The p h y l o g e n e t ic r e lationships a nd e x p re ss i on p r o f i l ing of C cHsf g e n e s under heat-stress w as studied. Phylogenetic analysis showed that CcH s f g e n e s w e r e di s t r i b ut e d i n to eight g r o u p s. I n th i s st u d y , c lasses A, B, and C were f u r ther subdivi d e d in t o subcl a sses such a s A1, A2, A 3, A4, A5, A6, A8, A 9, B1, B 2, B 3 , B 4 and C1. E x p r e ss i on p r o f ili n g of all 14 genes w a s ca r r i e d out by s e m i - q u a nt i ta t ive P CR, among which C c H sfA - 1d and C c HsfA - 2 w e r e observed to be highly upregulated duri n g heat-stress . R e l a t i ve qu a nt i f i ca t i on with qRT- P C R showed that C cHsfA - 1d i s up r e g ul a t e d 2 - 6 h r s af t e r heat-stress indicating its significant role as an early response factor. Our study provides a glimpse of the Hsf gene family in pigeonpea and this information can be utilized to gain more insight into the heat-response mechanism in pigeonpea. Abbreviations: AHA - Aromatic/ Hydrophobic /Acidic; Hsf - Heat Shock Factors; IRRK- Isoleucine, Arginine, Arginine, Lysine; NLS - Nuclear Localization Signal; NES - Nuclear Export Signal
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it