Integration and Expression Stability of Transgenes in Hybriding Transmission of Transgenic Rice Plants Produced by Particle Bombardment
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Four transgenic rice lines TR 5, TR 6, Ming B and Jingyin 119 obtained via particle bombardment were used as transgene donors to create hybrids. The integration and expression stability of exotic bar and cecropin B gene in conventional hybriding transmission were investigated by Southern and Northern blotting analyses. The selection marker bar gene was transferred to all hybrids under selection of Basta herbicide. Loss or gain of small hybridization bands (no more than 2.0 kb) of bar gene occurred in some hybrids, but the difference in integration sites of bar gene copies did not influence their stable expression. The non-selection gene cecropin B was stably transferred from the four transgene donors to their resulting hybrids, but expression level was very different. Silencing of cecropin B gene occurred in some hybrids from TR 5, TR 6 and Ming B. In the transgene donor Jingyin 119 and all its resulting hybrids, cecropin B and bar gene were stably expressed. We concluded that the stability of transgene during crossbreeding transmission is mainly determined by the primary transgenic donors and may be affected by recombination.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it